After a spun hematocrit test is completed, which of the following can be evaluated?

• A. Total protein, buffy coat, plasma color, and PCV
• B. PCV
• C. Plasma color and PCV
• D. Total protein, buffy coat, plasma color, fibrin, and PCV

Answer: (A)

Spun hematocrit separates blood into three visible portions: the bottom layer of packed red cells, the middle buffy coat of white blood cells and platelets, and the top plasma. This setup lets you assess multiple parameters beyond the hematocrit value. The PCV is read from the height of the red cell layer, giving the packed cell volume. The plasma color can indicate lipemia, icterus, or hemolysis, providing clues about metabolic or processing issues. The buffy coat thickness offers a qualitative look at the white cell and platelet content. The plasma can also be used to measure total protein with a refractometer. Fibrin would indicate clotting and is not a typical, reliable part of a standard spun hematocrit result. Therefore, you can evaluate total protein, buffy coat, plasma color, and PCV.