To separate serum, whole blood should be spun down in a centrifuge at:

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Study for the VTNE Laboratory Procedures Test. Use flashcards and multiple choice questions, each question offers hints and explanations, enabling increased understanding and retention. Prepare effectively for your exam!

Multiple Choice

To separate serum, whole blood should be spun down in a centrifuge at:

Explanation:
Centrifugation uses density differences to separate serum from the clot and cells. After the blood has clotted, spinning it helps push the heavier cellular components and clot remnants to the bottom, leaving clear serum on top for testing. The best setting provides enough centrifugal force to compact the cells and clot quickly but not so much force or for so long that it risks hemolysis or disrupting the clot. A moderate speed for a short duration achieves clean separation and preserves serum integrity, which is why this approach is preferred. Settings that are much higher or much longer can cause damage or incomplete separation, while too little force or too short a time may leave cells or clot still mixed with the serum. Keep in mind that the exact force depends on rotor size, but the principle is to balance adequate separation with sample safety.

Centrifugation uses density differences to separate serum from the clot and cells. After the blood has clotted, spinning it helps push the heavier cellular components and clot remnants to the bottom, leaving clear serum on top for testing. The best setting provides enough centrifugal force to compact the cells and clot quickly but not so much force or for so long that it risks hemolysis or disrupting the clot. A moderate speed for a short duration achieves clean separation and preserves serum integrity, which is why this approach is preferred. Settings that are much higher or much longer can cause damage or incomplete separation, while too little force or too short a time may leave cells or clot still mixed with the serum. Keep in mind that the exact force depends on rotor size, but the principle is to balance adequate separation with sample safety.

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